CAS Number
Formula
Weight
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Principle Iron sulphite agar is composed of tryptone, sodium sulphite, Ferric citrate and agar. Tryptone provides nitrogen and other trace nutrients for the growth of bacteria. Sodium sulphite is selective agent and sulphite source. Ferric citrate is sulphite reduction indicator. The sulphite reducing bacteria reduce sulphite to sulphide, which react with iron salt and forms black color complex. Since the sulphite reducing bacteria forms black color colonies. Agar is solidifying agent. |
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Use: Recommended for detection of anaerobic sulphide spoilage causing microorganism in food. |
Ingredients:
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Tryptone |
10,00 g/L |
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Sodium Sulphite |
0,500 g/L |
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Ferric citrate |
0,500 g/L |
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Agar |
15,00 g/L |
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pH at 25°C |
7,1±0,2 |
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Formula adjusted for optimum performance and parameters |
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Directions: Dissolve 26,00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 50-52 °C and distribute aseptically in test tubes (10 ml). Inoculate 1ml of test sample in molten state mix well. Allow to set the medium in up write position and incubate at desired temperature.
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Storage and Shelf Life: The product is highly hygroscopic; keep the container tightly closed at all times and store it properly as per the conditions mentioned on the label. The declared expiry is valid only when stored as per the conditions mentioned on the label. Note: Sterilize media immediately after reconstitution. |
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Disposal: To avoid the contamination or propagation of any hazardous microbes the used, unusable or modified preparation of this product must be disposed after autoclaving after completion of task. |
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